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仪器简介:快速动力学停流装置与光谱仪器配合进行快速动力学瞬间实时记录,或离线分析,为广大的快速动力学研究学者提供了强有力的武器。选择不同的配置可进行下列应用:
* 蛋白质折叠动力学(Protein Folding)
* 酶动力学(Enzyme Kinetice)
* 底物结合(Substrate Binding)
* 构象转变(Conformational Changes)
* 胞内底物运输(Substrate Transport in Vesicles)
* 第二信使研究(Second Messenger Studies)
* 快速化学反应动力学(Rapid Chemical Reaction Kinetics)
KinTek has always been aleader in building stopped-flow instrumentsthat conserve precious samples. The all new AutoSF-120 sets a new standard for sample economy, requiringonly 120 microliters of sample to flush, load and collect data, while giving with 3-4 replicate measurements.The flushing and sample loading areall done automatically. Simply pipette 120 microliters of solution into each sample cup and click "Load".
In the process of designing this new state-of-the-art instrument, we also added new noise reduction electronic circuits to improve signal:noise twofold and achieved asubmillisecond dead time. In addition we completely re-wrote the system software for data collection and analysis to give unparalleled ease of use and power.
The KinTek AutoSF is by far the best stopped-flow instrument ever built, giving you more high quality data while consumingless sample and less time.
An optionalrobotic autosamplerincreases throughput even more to afford unattended stopped-flow measurements ofhundreds of samples per day.
技术参数:
1.注射器数量:3个
2.驱动方式:每一个注射器由一个步进马达驱动
3.*小死时间:0.6毫秒(FC-08池)0.25毫秒(微量池)
4.注射流量范围:0.01-10ml/s(取决于安装的注射器)
5.混合比例范围:1/1至1/100连续可调
主要特点:
1.恒温水夹套设计,全电脑控制操作
2.程控驱动注射器,每路样品流量可调,不受浓度与粘度影响
3.高精度,完全可调的混合与稀释比例
4.极短的死时间,*短为0.25毫秒
5.二路、三路、四路流动注射停流装置可选择
Small sample volume:120 μL is sufficientto flush and load a new sample and get3-4 reproducible shots.All other instruments require 3-5 times as much sample.
Automated computer-controlledload/flush/fire cycle — simply pipet 120 μL into each sample cup and click "load".
Twofold improvement in signal/noise with new noise reduction circuits
Compact footprint: instrument and electronics combined (14 × 26.5 × 4.5 inches)
Computer optimized optical path with lower noise and background fluorescence
Automated sample loader, which can be interfaced with an optional robot.
200 μl of sample is sufficient to load and collect 6-8 good shots.
Standard 10 μl flow cell.
20 μl per shot per solution.
Hastwo fluorescence channelsandone absorbance channelthat can be observed and recorded simultaneously.
Under complete computer control, courtesy of the KinTek StopFlow Software.